Phosphomannose-isomerase as a selectable marker to recover transgenic orchid plants (Oncidium Gower Ramsey)
نویسندگان
چکیده
منابع مشابه
Phosphomannose isomerase, pmi, as a selectable marker gene for durum wheat transformation
Transgene technology provides a powerful tool for developing traits that are otherwise difficult to achieve through conventional methods. The development of transgenic plants requires the use of selectable marker genes, as the efficiency of plant transformation is less than optimal for many important species, especially for monocots such as durum wheat (Triticum turgidum var. durum). Many conce...
متن کاملMannose-Based Selection with Phosphomannose- Isomerase (PMI) Gene as a Positive Selectable Marker for Rice Genetic Transformation
A positive selectable marker system was adapted for transformation of mature embryo-derived calli of Indica rice (Oryza sativa L.) utilizing the PMI gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. The transformed cells grew on medium supplemented with 3% mannose as carbon source and calli were selected on media containing various concentrati...
متن کاملSelectable marker genes in transgenic plants: applications, alternatives and biosafety.
Approximately fifty marker genes used for transgenic and transplastomic plant research or crop development have been assessed for efficiency, biosafety, scientific applications and commercialization. Selectable marker genes can be divided into several categories depending on whether they confer positive or negative selection and whether selection is conditional or non-conditional on the presenc...
متن کاملPhosphomannose Isomerase, A Novel Selectable Plant Selection System: Mode of Action and Safety Assessment
Phosphomannose isomerase (PMI), an enzyme not present in many plants, catalyzes the reversible interconversion of mannose 6-phosphate and fructose 6-phosphate. Plant cells lacking this enzyme are incapable of surviving on synthetic medium containing mannose. Plants derived from sugar beet and corn cells that had been genetically modified to express the E. coli manA gene encoding PMI were evalua...
متن کاملFactors Affecting Synonymous Codon Usage Bias in Chloroplast Genome of Oncidium Gower Ramsey
Oncidium Gower Ramsey is a fascinating and important ornamental flower in floral industry. In this research, the complete nucleotide sequence of the chloroplast genome in Oncidium Gower Ramsey was studied, then analyzed using Codonw software. Correspondence analysis and method of effective number of codon as Nc-plot were conducted to analyze synonymous codon usage. According to the correspondin...
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ژورنال
عنوان ژورنال: Plant Cell, Tissue and Organ Culture (PCTOC)
سال: 2010
ISSN: 0167-6857,1573-5044
DOI: 10.1007/s11240-010-9827-0